What is the main subject of "The Last Supper" by Leonardo da Vinci?

The primary focus of "The Last Supper" by Leonardo da Vinci is indeed the final meal that Jesus shared with his disciples before his crucifixion. This pivotal moment in the Christian tradition illustrates the depth of the relationship between Jesus and his disciples, as well as the significance of Jesus’ impending sacrifice. The painting captures the emotional responses of each disciple as Jesus reveals that one of them will betray him. Da Vinci's composition, use of perspective, and attention to human emotion vividly convey the tension and intimacy of this moment.

In contrast, the other options pertain to different events in the life of Jesus that are not the focus of this particular work. The resurrection, birth, and ascension of Jesus are significant moments in Christian theology but are not depicted in this painting. "The Last Supper" specifically explores the themes of betrayal, friendship, and communion, making it a central event that underscores the narrative of the Passion of Christ, thus affirming that the correct answer highlights the essence of the artwork.